HPTLC Method for the Simultaneous Estimation of Tizanidine and Rofecoxib in Tablets
نویسندگان
چکیده
A new simple, accurate and precise HPTLC method has been developed for the simultaneous estimation of tizanidine and rofecoxib in tablet formulation. In this method, standard and sample solutions of tizanidine and rofecoxib were applied on precoated silica gel G 60 F254 TLC plate, and developed using nbutyl acetate: formic acid: chloroform (6:4:2 v/v/v), as mobile phase. The drugs on plate were scanned at 315 nm. The dynamic linearity range was 2-10 μg/spot for tizanidine and 16-80 μg/spot for rofecoxib. The method was validated for precision, accuracy, and reproducibility.
منابع مشابه
Recent progress in simultaneous estimation of rutin, quercetin and liquiritin in Cocculus hirsutus by HPTLC
Background and objectives: Rutin, quercetin and liquiritin arepolyphenol flavonoids which have shown anti-inflammatory, antihepatotoxic, antiulcer, antiallergic, antidiabetic, antiviral and antioxidant activities. They are found in many herbal plants, one of them is Cocculus hirsutus. The objective of this research was to develop and validate a new, accurate, precise and economic HPTL...
متن کاملSimultaneous estimation of amlodipine besylate and nebivolol hydrochloride in pharmaceutical tablets formulation by RP-HPLC using PDA detector
Background: The present work was undertaken with the aim to develop and validate a rapid and consistent RP-HPLC method in which the peaks will be appear with short period of time as per ICH Guidelines. Materials and Methods: The separation was achieved on a a stainless steel analytical column, Eclipse XDB plus C18 column (4.6 X 150 mm; 5 μm) in an isocratic mode. The mobile phase w...
متن کاملApplication of hydrotropic solubilization in spectrophotometric analysis of Esomeprazole and Itopride pharmaceutical combined tablet dosage forms
Two simple, accurate and precise methods for simultaneous estimation of Esomeprazole and Itopride in bulk drug and tablet dosage form have been described. Method-A employs formation and solving of simultaneous equation using 231 and 370 nm as two analytical wavelengths. Method -B is Absorption ratio method, which uses 308 and 331 nm as two analytical wavelengths.Beer’s law was obeyed in the con...
متن کاملHPTLC-densitometry method development and validation for simultaneous determination of abacavir, lamivudine and zidovudine in combined dosage form
A simple, accurate and precise HPTLC- densitometry method for the simultaneousdetermination of abacavir, lamivudine and zidovudine in pharmaceutical tablet has beendeveloped. Separation of tablet components was performed on aluminum backed HPTLC plates(silica gel 60 F254 20×20 cm with 0.2 mm thickness, Camag, Muttenz, Switzerland ) usingmethanol : chloroform : acetonitrile (4 : 8 : 3 v/v). Good...
متن کاملArtificial Neural Networks (ANN) for the simultaneous spectrophotometric determination of fluoxetine and sertraline in pharmaceutical formulations and biological fluid
Simultaneous spectrophotometric estimation of Fluoxetine and Sertraline in tablets were performed using UV–Vis spectroscopic and Artificial Neural Networks (ANN). Absorption spectra of two components were recorded in 200–300 (nm) wavelengths region with an interval of 1 nm. The calibration models were thoroughly evaluated at several concentration levels using the spectra of synthetic binary mix...
متن کامل